A Continuous Glucose Telemetry Solution for Mice and Rats
The ability to collect continuous glucose, temperature, and activity in a mouse or rat is possible through the use of DSI's PhysioTel Small Animal Telemetry system. A typical glucose system is comprised of 4-16 implants, 4-16 receivers, 1-2 matrices, and 1 computer running acquisition and analysis software.
How the system works
- An implanted mouse or rat resides in its home cage which is positioned on top of a DSI receiver.
- The implant continuously measures glucose and temperature and transmits those values out in real time.
- The receiver continuously listens to the transmitted signals and passes them along to the matrix.
- The matrix calculates a gross motor activity count for each receiver based upon observed signal strength characteristics. This activity data along with that receivers glucose and temperature data is then passed on to a computer running acquisition and analysis software.
- The acquisition portion of the software then records the data from all animals.
- The analysis portion of the software both analyzes incoming data in real time and can be used post-acquisition to refine the analysis based upon user inputs.
- An independent glucose measurement device such as a glucometer is also required for periodic in-vivo calibration of the implant. Glucose measurements from that reference device can be entered into the software either in real-time or during later analysis.
PhysioTel and PhysioTel HD System Hardware
The HD-XG functions with the PhysioTel and PhysioTel HD telemetry platform using the following hardware:
- PhysioTel® Receivers provide reliable reception of data transmitted via telemetry.
- The RPC-1 is most often used for monitoring mice and rats individually housed in plastic cages. Each cage is placed on top of a single RPC-1.
- The RSC-1 is a smaller profile receiver compared to the RPC-1 and is designed for use in special applications; for example, when running wheels or treadmills are part of the study design.
- The RPC-3 is most often used when pair housing small animal in plastic cages. This receiver is specific to 455 kHz frequency and 18 MHz frequency implants.This receiver may also be used when implanting two implants in one subject.
- Matrix 2.0 (MX2) manages the communication between the PhysioTel and PhysioTel HD implants, receivers, and the acquisition computer.
- Computer with appropriate software installed
- Networking Hardware enables the various pieces of hardware and software to communicate
- Router - used to assign IP addresses.
- Switch with PoE (Power over Ethernet) - used to power the hardware and allow multiple connections to the network.
Note: This same system supports all of our other small animal telemetry implants. In cases where a pressure measurement is made an ambient pressure reference called an APR-2 is also required (and shown in the above diagram).
HD-XG continuous glucose telemetry implants are supported in Ponemah version 6.33 and higher*
DSI recognizes that some researchers interested in small animal glucose measurements desire or require GLP compliance to FDA 21 CFR Part 11 and we expect to offer such a software solution in the future.
*The HD-XG implant is also compatible with LabChart from AD Instruments.
An essential webinar for researchers interested in collecting a complete glucose profile via implantable telemetry to minimize animal stress and improve animal welfare. Watch it Now