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中枢神经学是探索人类或动物模型的神经系统如何发育、 组织管理、和产生行为等功能的科学。Tools 可作为研究解剖学、生理学、生物化学、或神经和神经组织分子生物学的有力工具。(摘自神经科学第四版第一页)

神经细胞,或神经元,组成神经通路从而接收、处理和传输神经电和化学信号。 电生理记录仪可通过在感兴趣的神经细胞附近放置电极监测这些神经通路的电活动。主要参数如下:

  • 单个神经元动作电位 (峰值)
  • 潜在低频区域(LFP)
  • 脑电(EEG)或脑电波
  • 肌电(EMG) 或肌肉活跃度
  • 眼电 (EOG)或 眼球运动
  • 外周交感神经活动 (SNA)
  • 活跃期/镇静期
  • 行为学

通过监测神经通路的电活动,可获取大量信息,帮助了解神经系统功能、寻求多种神经或精神系统疾病的预防或治疗方案比如:睡眠状况、癫痫或癫痫症、创伤性脑损伤、行为学、神经退行性疾病等

DSI提供多种中枢神经系统解决方案,可监测束缚状态下或清醒自由活动状态下的实验动物模型。我们的高质量的产品性能优良、可最大程度满足您的科研需求。


DSI中枢神经系统研究解决方案


DSI提供强大的神经系统研究解决方案,通过植入式遥测或传统连线式监测方法可监测多种动物模型,为您
采集和分析单一或多通道生理信号。

植入式遥测


DSI PhysioTel™ 系列植入子适用于监测清醒、自由活动的实验动物生理信号,为您提供最自然状态下实验动物的真实数据。PhysioTel 植入子有多种型号,适用于不同动物种属:从小鼠、大鼠、到犬或猴等。植入子尺寸的设计根据不同的手术植入位置,适用于皮下植入或腹腔植入等。

DSI提供多种型号和功能的植入子,满足研究者监测不同生理参数的需求。以下表格标明了不同型号植入子为中枢神经系统研究提供的多种选择。

 

物种


可监测的生物电信号通道数量

1

2

3

4

XS (小鼠)

ETA-F10, ETA-F20, EA-F20, HD-X11

F20-EET

SA (大鼠, 豚鼠)

CA-F40, CTA-F40, HD-S11, HD-S21

F40-EET

F50-EEE

4ET

LA (犬, 猴)

CTA-D70, D70-PCT, D70-PCTP, L21, L11

D70-CCTP

D70-EEE

 

ACQ7700生理信号监测平台

DSI提供优质的、操作简易的的连线式生理信号监测系统,可对多种束缚状态下的实验动物进行短期监测,满足研究者需采集高通量、高频宽生理参数的需求。通用生理信号采集平台可采集单通道或多通道生物电信号。

DSI ACQ7700生理信号监测系统提供多种必要组件帮助研究者采集中枢神经系统相关生理参数,为研究者倾向于使用的电极配置提供监测系统接口平台。

 

 

软件 – 数据采集

 

DSI提供多种软件平台帮助您进行中枢神经系统研究,如果同步行为学研究相关参数对您的研究有所帮助,Ponemah™ 软件平台可提供同步视频功能。

如果您倾向于用第三方系统采集数据,我们可为您提供大部分中枢神经系统研究相关植入子的模拟信号输出解决方案。


软件 – 数据分析

 

DSI NeuroScore™ 软件是针对中枢神经系统专门开发的分析软件,可对中枢神经系统生理信号进行专业、高效的数据分析。模块化的分析平台可提供自动睡眠分析、癫痫检测、视频同步模块和批量处理模块。以下列表总结了应用NeuroScore软件常见的典型案例:

小动物睡眠分析模块

  • 可分析双通道脑电信号,单通道肌电信号,以及活动度信号等
  • 可自动识别慢波睡眠期 (SWS),快动眼睡眠期,清醒期和活跃期等

 

大动物睡眠分析模块

  • 可分析单通道脑电信号、单通道眼电信号、单通道肌电信号、以及活动度等。
  • 可自动识别非快动眼睡眠期(N1, N2, N3)、快动眼睡眠期、清醒期和活跃期等

 

癫痫检测模块

  • 通过脑电波振幅阈值和其它峰值特性识别峰值和爆发波

DSI中枢神经系统研究的每套系统都由传感器、硬件、软件和相关配件组成,为您提供精准的实验数据和可靠实验结果。

 

DSI中枢神经系统研究解决方案常用系统配置如下:

小动物植入式遥测系统




大动物植入式遥测系统 (PhysioTel Digital)





植入式遥测系统 – 模拟信号输出至第三方软件系统




ACQ7700生理信号监测系统


Many scientific articles have been published using DSI system for neuroscience research applications. The following is a selection of articles listed by species.

Mouse

Tang X, Sanford LD.  “Telemetric recording of sleep and home cage activity in mice.”  Sleep. 2002 Sep 15;25(6):691- 9.

Weiergräber M, Henry M, Hescheler J, Smyth N, Schneider T.  “Electrocorticographic and deep intracerebral EEG recording in mice using a telemetry system.”  Brain Res Brain Res Protoc. 2005 Apr;14(3):154-64.

Zhang S, Zeitzer JM, Sakurai T, Nishino S, Mignot E.  “Sleep/wake fragmentation disrupts metabolism in a mouse model of narcolepsy.”  J Physiol. 2007 Jun 1;581(Pt 2):649-63.

Glickstein SB, Moore H, Slowinska B, Racchumi J, Suh M, Chuhma N, Ross ME.  “Selective cortical interneuron and GABA deficits in cyclin D2-null mice.”  Development. 2007 Nov;134(22):4083-93.

Olivadoti MD, Opp MR.  “Effects of i.c.v. administration of interleukin-1 on sleep and body temperature of interleukin-6-deficient mice.”  Neuroscience. 2008 Apr 22;153(1):338-48.

Ahnaou A, Dautzenberg FM, Geys H, Imogai H, Gibelin A, Moechars D, Steckler T, Drinkenburg WH.  “Modulation of group II metabotropic glutamate receptor (mGlu2) elicits common changes in rat and mice sleep-wake architecture.”  Eur J Pharmacol. 2009 Jan 28;603(1-3):62-72.

Colic S, Wither R, Eubanks JH, Zhang L, Bardakjian BL.  “EEG analysis for estimation of duration and inter-event intervals of seizure-like events recorded in vivo from mice.”  Conf Proc IEEE Eng Med Biol Soc. 2011;2011:2570-3.

El-Hayek YH, Wu C, Chen R, Al-Sharif AR, Huang S, Patel N, Du C, Ruff CA, Fehlings MG, Carlen PL, Zhang L.  “Acute postischemic seizures are associated with increased mortality and brain damage in adult mice.”  Cereb Cortex. 2011 Dec;21(12):2863-75.

Beamer E, Otahal J, Sills GJ, Thippeswamy T. “N (w) -propyl-L-arginine (L-NPA) reduces status epilepticus and early epileptogenic events in a mouse model of epilepsy: behavioural, EEG and immunohistochemical analyses.”  Eur J Neurosci. 2012 Nov;36(9):3194-203.

Machida M, Yang L, Wellman LL, Sanford LD.  “Effects of stressor predictability on escape learning and sleep in mice.”  Sleep. 2013 Mar 1;36(3):421-30.  

Rat

Bastlund JF, Jennum P, Mohapel P, Vogel V, Watson WP.  “Measurement of cortical and hippocampal epileptiform activity in freely moving rats by means of implantable radiotelemetry.”  J Neurosci Methods. 2004 Sep 30;138(1-2):65-72.

Williams P, White A, Ferraro D, Clark S, Staley K, Dudek FE.  “The use of radiotelemetry to evaluate electrographic seizures in rats with kainate-induced epilepsy.”  J Neurosci Methods. 2006 Jul 15;155(1):39-48.

Beig MI, Bhagat N, Talwar A, Chandra R, Fahim M, Katyal A.  “Simultaneous recording of electroencephalogram and blood pressure in conscious telemetered rats during ictal state.”  J Pharmacol Toxicol Methods. 2007 Jul-Aug;56(1):51-7.

Kadriu B, Guidotti A, Costa E, Auta J. “Imidazenil, a non-sedating anticonvulsant benzodiazepine, is more potent than diazepam in protecting against DFP-induced seizures and neuronal damage.” Toxicology. 2009 Feb 27;256(3):164-74.

Kinkead R, Montandon G, Bairam A, Lajeunesse Y, Horner R.  “Neonatal maternal separation disrupts regulation of sleep and breathing in adult male rats.”  Sleep. 2009 Dec;32(12):1611-20.

Moscardo E, Rostello C.  “An integrated system for video and telemetric electroencephalographic recording to measure behavioural and physiological parameters.”  Journal of Pharmacological and Toxicological Methods.  2010;62(1):64-71.
 
Wasterlain CG, Stöhr T, Matagne A.  “The acute and chronic effects of the novel anticonvulsant lacosamide in an experimental model of status epilepticus.”  Epilepsy Res. 2011 Mar;94(1-2):10-7.

Diack C, Ackaert O, Ploeger BA, van der Graaf PH, Gurrell R, Ivarsson M, Fairman D.  “A hidden Markov model to assess drug-induced sleep fragmentation in the telemetered rat.”  J Pharmacokinet Pharmacodyn. 2011 Dec;38(6):697-711.
 
Langston JL, Wright LK, Connis N, Lumley LA.  “Characterizing the behavioral effects of nerve agent-induced seizure activity in rats: increased startle reactivity and perseverative behavior.”  Pharmacol Biochem Behav. 2012 Jan;100(3):382-91.

Seke Etet PF, Palomba M, Colavito V, Grassi-Zucconi G, Bentivoglio M, Bertini G.  “Sleep and rhythm changes at the time of Trypanosoma brucei invasion of the brain parenchyma in the rat.”  Chronobiol Int. 2012 May;29(4):469-81.

De Araujo Furtado, Marcio, et al. "Spontaneous recurrent seizures after status epilepticus induced by soman in Sprague‐Dawley rats." Epilepsia, 51.8 (2010): 1503-1510.


White, Andrew M., et al. "Efficient unsupervised algorithms for the detection of seizures in continuous EEG recordings from rats after brain injury."
Journal of Neuroscience Methods,152.1 (2006): 255-266.

Katalan, Shahaf, et al. "Magnesium sulfate treatment against sarin poisoning: dissociation between overt convulsions and recorded cortical seizure activity." Archives of Toxicology, 87.2 (2013): 347-360.

Guinea Pig

Mumford H, Wetherell JR.  “A simple method for measuring EEG in freely moving guinea pigs.”  J Neurosci Methods.  2001 May 30;107(1-2):125-30.

Joosen MJ, van der Schans MJ, van Helden HP.  “Percutaneous exposure to the nerve agent VX: Efficacy of combined atropine, obidoxime and diazepam treatment.”  Chem Biol Interact. 2010 Oct 6;188 (1):255-63.

Mumford H, Troyer JK.  “Post-exposure therapy with recombinant human BuChE following percutaneous VX challenge in guinea-pigs.”  Toxicol Lett. 2011 Sep 25;206(1):29-34.

Wang Y, Oguntayo S, Wei Y, Wood E, Brown A, Jensen N, Auta J, Guiodotti A, Doctor BP, Nambiar MP.  “Neuroprotective effects of imidazenil against chemical warfare nerve agent soman toxicity in guinea pigs.”  Neurotoxicology. 2012 Mar;33(2):169-77.

Non-human Primate

Pearce PC, Crofts HS, Muggleton NG, Ridout D, Scott EA.  “The effects of acutely administered low dose sarin on cognitive behaviour and the electroencephalogram in the common marmoset.”  J Psychopharmacol. 1999;13(2):128-35.

Authier S, Paquette D, Gauvin D, Sammut V, Fournier S, Chaurand F, Troncy E.  “Video-electroencephalography in conscious non human primate using radiotelemetry and computerized analysis: refinement of a safety pharmacology model.”  J Pharmacol Toxicol Methods. 2009 Jul- Aug;60(1):88-93.

Barraud Q, Lambrecq V, Forni C, McGuire S, Hill M, Bioulac B, Balzamo E, Bezard E, Tison F, Ghorayeb I.  “Sleep disorders in Parkinson's disease: the contribution of the MPTP non-human primate model.”  Exp Neurol. 2009 Oct;219(2): 574-82. 

Dog

Dürmüller, Niklaus, Philippe Guillaume, Pierre Lacroix, Roger D. Porsolt, and Paul Moser. "The use of the dog electroencephalogram (EEG) in safety pharmacology to evaluate proconvulsant risk." Journal of Pharmacological and Toxicological Methods 56, no. 2 (2007): 234-238.


This information is provided in good faith and believed accurate at the time of writing. No responsibility will be taken, or liability accepted, for damages arising from the use of information herein.